Abstract: The DDI-Chip is a unique and easy-to-use patented microfluidic device with five interconnected channels capable of reconstructing complex biological microenvironments for a wide range of applications and analyses. The company also offers microfluidic chip processing services in a variety of materials and configurations.
The DDI-Chip is a microfluidic device by which interactions between cells and factors can be investigated at different distances. This provides a more physiologic setup and allows different distances to be investigated simultaneously in the same device. Using the DDI-Chip, it is also possible to test for agents that may affect distance-related interactions.
This package of 3 DDI Chips is sterile, and enclosed in a petri dish. The chips must be stored in dry conditions, not exposed to direct sunlight, and at room temperature (15-25 °C).
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The DDI-Chip is made of biocompatible polydimethylsiloxane (PDMS), which is breathable and ensures CO2 and O2 exchange. The support in the thin glass is a microscope slide with excellent optical properties such as high transparency and low autofluorescence. This allows direct observation of your biological samples with temporal and spatial resolution using optical, fluorescence and confocal microscopy.
The model includes:
- 2 media channels (external channels) for nutrient supply and test compound injection;
- 2 substrate channels (intermediate channels) for cell inoculation with or without hydrogel;
- 1 main central corridor, the width of which increases gradually along its length
| side channel | Intermediate Matrix Channel | corridor | |
| Height | 150μm | 150μm | 150μm |
| Width | 2500μm | 1125 µm (narrow portion) 3375 µm (wide portion) | Gradual change from 1250 µm to 5000 µm |
| 体积（建议注射） | 20μl | 20μl | 20μl |
| 入口直径 | 1000μm | 1000μm | 1000μm |
DDI-Chip has an uncoated surface. Depending on your application and to ultimately improve cell adhesion, you can use polymer and/or protein solutions to coat the channel surface.
This microfluidic device can also be used with cells embedded in hydrogels (e.g., collagen I, collagen IV, fibronectin, matrix gel, etc.) to re-establish a reliable biological microenvironment.
The 1 mm inlet diameter allows for easy injection of cell suspensions through a common pipette. 20 µL is the recommended volume per channel/chamber; this will allow for a few extra µL during the actual injection and complete fill.
By using 1/16″ tubing mounted directly in the inlet, studies can be conducted under dynamic flow conditions.
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