Microfluidic cell sorting chip

Microfluidic Cell Sorting Chip Overview

A microfluidic cell sorting chip is a chip prepared using microfluidics and micro- and nanofabrication technologies for precise separation and sorting of mixed cells or microorganisms.

This technology allows manipulation and separation of cells by fluid control in microchannels with high efficiency, high throughput, low cost, and low sample consumption.

The structure of microfluidic cell sorting chip is mainly composed of flow channel, control system and detection system. Among them, the flow channel is the most critical part of the chip, and by designing different structures and sizes, functions such as separation, manipulation and aggregation of cells can be realized.

The control system, on the other hand, enables the localization and manipulation of cells by controlling the flow and pressure of microfluidics in the flow channel. The detection system detects and analyzes the separated cells, such as cell counting, sorting and single cell analysis.

Microfluidic cell sorting chip has a wide range of application prospects in the fields of biomedicine, bioengineering and environmental monitoring. For example, in the field of biomedicine, it can be used in the separation of tumor cells, early diagnosis of cancer, drug screening and so on;

In the field of bioengineering, it can be used in the isolation and screening of microorganisms, metabolic engineering and so on; in the field of environmental monitoring, it can be used in the monitoring and detection of microorganisms in water quality and air and so on.

Experimental Methods for Cell Sorting

Below are the steps for a general cell sorting chip experiment:

1、Sample preparation: The cells to be sorted are processed and prepared, such as colloidal gold staining, cell lysis, centrifugation and so on. Sample treatment should be carried out according to specific experimental requirements to ensure cell viability and quality.

2, chip processing: the cell sorting chip will be processed, such as cleaning, disinfection, removal of air bubbles, etc., to ensure that the chip is sterile and free from contamination.

3, determine the chip sorting conditions: determine the chip sorting flow rate, flow channel size and sorting conditions and other parameters. The setting of these parameters should be optimized according to the experimental needs and the characteristics of the sample.

4、Load sample: The processed cell sample is injected into the microfluidic channel through the sample inlet in the microfluidic chip.

5, cell sorting: according to the experimental design and sorting conditions, cell sorting is carried out on the cell sorting chip. In the process of sorting, the microfluidic system in the chip controls the direction and speed of cell flow and separates different types of cells.

6, Detection and analysis: Detection and analysis of the sorted cells. Commonly used methods include microscopic observation, fluorescent staining, cell counting, flow cytometry and so on.

7. Data analysis and interpretation of results: The experimental data will be statistically analyzed and the results interpreted to determine the effectiveness and accuracy of the sorting, as well as the potential and limitations of the application.

The above are the basic steps for general cell sorting chip experiments. In the experimental process, it needs to be optimized and adjusted according to the specific experimental requirements and the characteristics of the chip. At the same time, it is necessary to pay attention to the aseptic operation and safety of the chip and samples in the experimental process to ensure the reliability and accuracy of the experimental results.

Recent Research Progress and Development of Cell Sorting

Cell sorting technology has always been an important tool in cytology and biology research, and has been further developed and applied in recent years with the continuous development of microfluidics and bioimaging technologies. The following are the latest research progress and development directions:

1、Multiple cells sorting at the same time: traditional cell sorting technology can generally only separate a single type of cell, and it is difficult to sort many different types of cells at the same time. Recent studies have found that through microfluidic chip and fluorescence staining methods, multiple cells can be sorted at the same time, improving the efficiency and accuracy of sorting.

2、Single cell analysis: Single cell analysis technology can analyze and study single cells, but it has been limited due to small sample size and difficult operation. In recent years, through microfluidic chip and imaging technology and other methods, single cells can be analyzed in high throughput to achieve the sorting, detection and analysis of single cells.

3, cell dynamics research: cell dynamics is one of the important directions of cell biology research, which can be used to observe and analyze the biological processes inside cells in real time. The latest research shows that through microfluidic chip and fluorescence imaging technology and other methods, it is possible to carry out real-time observation and research on molecular movement, signal transduction and metabolic processes inside the cell.

4, cellular medicine applications: cell sorting technology has also been widely used in cellular medicine. For example, stem cells and cancer cells can be sorted and screened for therapeutic and diagnostic purposes through cell sorting technology. The latest research shows that through microfluidic chip and bio-imaging technology and other methods, high-throughput sorting and single-cell analysis of tumor cells can be achieved, which is expected to realize early diagnosis and treatment of cancer.

Overall, microfluidic cell sorting technology has a broad application prospect in cytology and biology research, and the future development direction includes the research and development of high-throughput, high-precision, high-speed, and single-cell analysis.

Cell Sorting Chip Related Papers

Below are a few classic cell sorting chip related papers and their brief overviews:

1、“Microfluidic Cell Sorter (MCS) for On-Chip Capture and Analysis of Single Cells” (Analytical and Bioanalytical Chemistry, 2011)

This article describes a microfluidic chip-based cell sorting technique capable of sorting and analyzing individual cells. The technology sorts cells individually into different microwells via microchannels and separation valves for subsequent analysis and detection.

2、“On-chip sorting of bacteria using inertial microfluidics” (Lab on a Chip, 2013)

This article describes a bacterial sorting chip based on inertial microfluidics that can sort and classify different types of bacteria. The technology utilizes centrifugal and inertial forces in microchannels to individually sort bacteria to different locations for subsequent analysis and detection.

3、“Continuous-flow microfluidic bioparticle concentrator” (Lab on a Chip, 2013)

This article describes a continuous flow-based microfluidic technique for sorting and concentrating biological particles. The technique utilizes deposition and migration forces in microchannels to individually sort biological particles to different locations for subsequent analysis and detection.

4、“Microfluidic device for single cell analysis” (Analytical Chemistry, 2015)

This article describes a microfluidics-based single-cell analysis chip that allows high-throughput analysis and detection of individual cells. The technology utilizes microchannels and separation valves to individually sort individual cells into different microwells for subsequent analysis and detection.

Overall, all of these papers demonstrate different microfluidic cell sorting techniques and provide important ideas and methods for the development and application of microfluidic cell sorting.

DXFLUIDICS

Dxfluidics is a specialized enterprise dedicated to customizing various microfluidic organ chips. With a highly skilled and experienced processing team, we offer one-stop organ chip processing services for our clients. The company is committed to providing high-quality, high-performance biochip products for the fields of biomedicine, biomedicine, bio-detection, cell culture, and more.

Let the flower of life bloom more beautifully, Dxfluidics microfluidic organ chips make miniature life experiments more precise!