Amino modification of biochip surfaces is a common modification method that imparts certain chemical activity and affinity to the surface by introducing amino groups to the surface. Amino modification can be used for applications such as control of surface chemistry, functionalization, and immobilization and detection of biomolecules.
The following is an overview of amino modifications on biochip surfaces:
1、氨基硅烷修饰:一种常见的氨基修饰方法是利用氨基硅烷化合物,如3-氨丙基三甲氧基硅烷(APTES),在生物芯片表面引入氨基基团。
APTES可以与表面的羟基或氧化物反应形成化学键,形成覆盖层,赋予表面氨基化性质。氨基硅烷修饰后的生物芯片表面可用于固定生物分子,如抗体、寡核苷酸等。
2、氨基化聚合物涂层:另一种常见的氨基修饰方法是利用氨基化聚合物涂层,如聚乙烯亚胺(PEI)、聚乙烯醇(PVA)等。
这些聚合物含有氨基基团,可以通过涂覆的方式将氨基功能引入生物芯片表面。氨基化聚合物涂层可以提供表面的化学活性,用于固定生物分子、调控细胞行为等应用。
3、氨基化金属表面:金属表面的氨基修饰通常通过在金属表面引入氨基化合物实现。
例如,将金属表面浸泡在含有氨基化合物的溶液中,或通过化学修饰方法在金属表面形成氨基化层。氨基化金属表面可以用于固定生物分子、制备生物传感器等应用。
4, Amino-chemical nanoparticle modification: Amino-chemical nanoparticles are a commonly used amino modification strategy to make nanoparticles chemically active and amphiphilic by introducing amino groups on their surfaces. Aminated nanoparticles can be used to immobilize biomolecules, prepare nanobiosensors, etc.
5、氨基化交联剂修饰:利用氨基化交联剂,如二甲二胺(DMEDA)、戊二胺(EDA)等,在生物芯片表面引入氨基基团。
这些交联剂可以与表面上的功能基团发生反应,形成交联网络,增加表面的化学活性和亲和性。氨基化交联剂修饰可用于生物分子的固定、生物传感器的制备以及控制表面的亲水性或疏水性。
6、氨基修饰的反应条件和控制:在进行生物芯片表面的氨基修饰时,需要注意合适的反应条件和控制。反应条件如溶剂选择、温度、pH值等可以影响修饰效果和稳定性。
此外,还可以通过控制修饰时间、修饰剂的浓度和修饰层的厚度等参数,来调节表面氨基化的程度和密度,以满足特定应用的需求。
7、氨基修饰后的功能化:氨基修饰后的生物芯片表面可以进一步进行功能化,通过引入其他化学基团实现更多的应用。
例如,可以利用氨基基团与羧基、醛基、酰胺基等反应,进行共价键的形成,引入更多的功能基团。这样可以实现对生物分子的特异性固定、表面化学反应的控制以及生物芯片的多功能性。
Overall, amino modifications on the surface of biochips provide them with chemical activity and affinity, extending their range of applications. Immobilization of biomolecules, modulation of biological interactions, and construction of biochips with specific functions can be achieved by selecting appropriate amination methods and further functionalization.
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