Amino modification of biochip surfaces is a common modification method that imparts certain chemical activity and affinity to the surface by introducing amino groups to the surface. Amino modification can be used for applications such as control of surface chemistry, functionalization, and immobilization and detection of biomolecules.
The following is an overview of amino modifications on biochip surfaces:
1, Aminosilane modification: A common method of amino modification is the use of aminosilane compounds, such as 3-aminopropyltrimethoxysilane (APTES), to introduce amino groups on the surface of the biochip.
APTES can react with hydroxyl groups or oxides on the surface to form a chemical bond, forming a covering layer that imparts aminosilane properties to the surface. The aminosilane-modified biochip surface can be used to immobilize biomolecules such as antibodies, oligonucleotides, etc.
2, aminated polymer coating: Another common method of amino modification is to utilize aminated polymer coatings, such as polyethyleneimine (PEI) and polyvinyl alcohol (PVA).
These polymers contain amino groups that can be coated to introduce amino functionality to the surface of a biochip. The aminated polymer coatings can provide chemical activity on the surface for applications such as immobilization of biomolecules and modulation of cell behavior.
3, aminated metal surfaces: Amino modification of metal surfaces is usually achieved by introducing amino compounds on the metal surface.
For example, the metal surface is immersed in a solution containing an amino compound, or an aminated layer is formed on the metal surface by a chemical modification method. The aminated metal surface can be used for applications such as immobilizing biomolecules and preparing biosensors.
4, Amino-chemical nanoparticle modification: Amino-chemical nanoparticles are a commonly used amino modification strategy to make nanoparticles chemically active and amphiphilic by introducing amino groups on their surfaces. Aminated nanoparticles can be used to immobilize biomolecules, prepare nanobiosensors, etc.
5、Amino-crosslinking agent modification: Use amino-crosslinking agents, such as dimethylenediamine (DMEDA), pentylenediamine (EDA), etc., to introduce amino groups on the surface of the biochip.
These cross-linkers can react with functional groups on the surface to form a cross-linking network, increasing the chemical activity and affinity of the surface. Aminated crosslinker modifications can be used for immobilization of biomolecules, preparation of biosensors, and control of hydrophilicity or hydrophobicity of surfaces.
6. Reaction conditions and control of amino modification: When carrying out amino modification on the surface of a biochip, it is necessary to pay attention to suitable reaction conditions and control. Reaction conditions such as solvent selection, temperature, pH, etc. can affect the modification effect and stability.
In addition, the degree and density of surface amination can be adjusted to meet the needs of a particular application by controlling parameters such as the modification time, the concentration of the modifier, and the thickness of the modified layer.
7. Functionalization after amino modification: The surface of biochip after amino modification can be further functionalized to achieve more applications by introducing other chemical groups.
For example, amino groups can be utilized to react with carboxyl, aldehyde, and amide groups to perform covalent bond formation and introduce more functional groups. This enables specific immobilization of biomolecules, control of surface chemical reactions, and versatility of biochips.
Overall, amino modifications on the surface of biochips provide them with chemical activity and affinity, extending their range of applications. Immobilization of biomolecules, modulation of biological interactions, and construction of biochips with specific functions can be achieved by selecting appropriate amination methods and further functionalization.
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An innovative company focusing on microfluidic chips and biochips, etc., is able to provide customized surface modification/modification services for microfluidic chips and biochips with the help of the company's new material surface coating processing technology. Our coating processing technology can enhance the surface properties of chips, improve their durability, corrosion resistance and biocompatibility, and realize specific functions, such as enhanced signal transmission, antimicrobial and cell adhesion control. By partnering with us, customers can gain access to advanced coating solutions in the field of microfluidic chips and biochips, opening up a wider range of possibilities for their research and applications.
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