Microfluidic Lung Organ Chip is a micro experimental platform designed and fabricated based on microfluidics technology to simulate the structure and function of lungs.
It is a micro-organ composed of a series of micro-channels, chambers and cell culture membranes that mimic physiological and pathological processes such as respiratory movements of the lungs, exchange of oxygen and carbon dioxide, and immune responses.
By introducing different types of cells, viruses, bacteria and drugs within the chip, it is possible to mimic lung diseases and screen drugs.
Microfluidic lung organ chips have many advantages, such as high reproducibility, high controllability, high simulation, easy manipulation and automation.
Compared to traditional cell culture and animal testing, microfluidic lung organoids offer higher accuracy and reliability, and can better mimic the physiology and pathology of the lungs.
Therefore, it can provide an efficient and reliable experimental platform for lung disease research, drug screening and toxicity assessment, and is expected to become an important tool for lung disease treatment and drug development.
The human alveolus chip includes a hollow side channel, allowing the application of suction to the chip, imposing cyclic strain mimicking normal human respiratory movements (left image). A permeable membrane separates human alveolar cells in the upper channel from human vascular cells in the lower channel, enabling them to exchange molecular signals (right image).
Experimental Methods for Lung Chip:
The microfluidic lung organ chip is a miniature experimental platform based on microfluidics technology, designed to simulate physiological and pathological processes of the lungs. Experiments on this chip can be conducted through the following methods:
- Design and Manufacture of Microfluidic Lung Organ Chip: Designers use CAD software or other simulation tools to draw the chip's structure and flow paths, then employ micro/nanofabrication techniques for chip manufacturing.
- Cell Culture: Lung cells (such as alveolar epithelial cells, pulmonary endothelial cells, etc.) are introduced into the chip and cultured under appropriate conditions to facilitate cell growth and differentiation.
- Simulation of Respiratory Movements: Using micro-pumps, the chip's respiratory movements are simulated by introducing gases into the chip, causing volume changes in alveoli and pulmonary vascular chambers to mimic human respiratory movements.
- Oxygen and Carbon Dioxide Exchange: Introducing appropriate gas mixtures into the chip allows the simulation of oxygen and carbon dioxide exchange within the chip. Control of gas flow and concentration replicates the exchange process in the lungs.
- Drug Screening and Toxicity Assessment: Different drugs or toxins are introduced into the chip, and cellular responses and physiological parameter changes within the chip are observed to evaluate drug efficacy and toxicity.
- Data Collection and Analysis: Integrated sensors and microscopy tools within the chip are used to collect experimental data. Data analysis tools are then employed to process and analyze the gathered data.
In summary, the microfluidic lung organ chip provides a highly realistic microenvironment for lung-related experiments in areas such as lung disease research, drug screening, and toxicity assessments.
Recent Advances in Lung Chip Research:
The microfluidic lung organ chip is a novel platform for in vitro research on the physiological and pathological aspects of the lungs. It can simulate the structure and function of the lungs and is capable of high-throughput drug screening and disease modeling experiments. In recent years, research progress in microfluidic lung organ chips has been rapid, primarily encompassing the following aspects:
- The application of bioprinting technology: Bioprinting technology can precisely position cells and biomolecules within microfluidic chips, thereby achieving a more realistic simulation of lung tissue structure and function.
- Simulating disease states: Researchers can use microfluidic lung organ chips to simulate lung diseases such as pulmonary fibrosis, pneumonia, and lung cancer. This allows for the study of corresponding pathophysiological mechanisms and the effects of drug treatments.
- High-throughput screening: Microfluidic lung organ chips enable high-throughput drug screening experiments, rapidly identifying drugs with potential therapeutic effects and accelerating the pace of new drug development.
- Integration of multiple functions: Microfluidic lung organ chips can integrate various functions, including gas exchange, blood supply, cell cultivation, and drug screening. This provides a more realistic simulation of the physiological and pathological states of the lungs.
In the future, research on microfluidic lung organ chips will delve deeper, serving not only for fundamental scientific research but also for clinical applications such as lung cancer screening and treatment.
The recommended literature for lung organ chips.
Here are translations of the provided text into English:
- "Microfluidic Lung Airway-on-a-Chip with Arrayable Suspended gels for Studying Epithelial and Smooth Muscle Cell Interactions” (2019): This paper introduces a microfluidic chip utilizing suspended gels to simulate the structure and function of human lung airways. The chip can be used to investigate the pathogenesis of lung diseases and drug screening.
2. “Microfluidic lung models to study alveolar epithelial barrier function” (2020): This literature proposes a lung model manufactured using microfluidic technology to study the function and disruption of alveolar epithelial barriers, as well as the pathogenesis of lung diseases. This research provides new experimental methods for lung drug screening and treatment.
3. “Microfluidic Organ-on-a-Chip Models of Human Lung Cancer” (2019): This paper introduces a lung cancer organ chip manufactured using microfluidic technology, which can be used to study the pathogenesis and treatment of lung cancer. The chip can simulate processes such as the growth, migration, and drug sensitivity of lung cancer cells in the lung microenvironment.
4.“Development of a microfluidic chip for pulmonary drug screening using A549 lung cancer cells” (2020): This literature describes a microfluidic chip for lung cancer drug screening, manufactured using microfluidic technology. It is used to assess the sensitivity and drug resistance of A549 lung cancer cells. The chip can simulate processes such as drug metabolism and the growth and migration of tumor cells in the lung microenvironment.
These papers showcase the application prospects of microfluidic lung organ chips in the research and drug screening of lung diseases. By simulating the lung's microenvironment and physiological processes, microfluidic lung organ chips offer an efficient and reliable experimental platform for the treatment and drug development of lung diseases.
Dxfluidics Products
| Product Code | Outline （mm） | Channel | Microporous membrane | Material | Price （CNY） | |||
| Height （mm） | Width （mm） | Space （mm） | Thickness （um） | Hole diameter （um） | ||||
| 600001 | 30*15 | 0.5 | 1 | 0.2 | 25 | 8 | PDMS | 800 |
| 600002 | 30*15 | 0.5 | 1 | 0.5 | 25 | 8 | PDMS | 800 |
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DXFLUIDICS
Dxfluidics is a specialized enterprise dedicated to customizing various microfluidic organ chips. With a highly skilled and experienced processing team, we offer one-stop organ chip processing services for our clients. The company is committed to providing high-quality, high-performance biochip products for the fields of biomedicine, biomedicine, bio-detection, cell culture, and more.
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