Technology in the biomedical field continues to innovate, and single-molecule RNA analysis microarrays (amplification-free) have attracted widespread attention as an advanced bioanalytical technology.
The amplification-free nature of this technology and its high sensitivity to individual RNA molecules, as compared to traditional RNA analysis methods, opens up entirely new possibilities for the early diagnosis and treatment of diseases.
This paper will provide insights into the technical principles, application areas, advantages, challenges, and future perspectives of single-molecule RNA analysis microarrays.
I. Technical Principles:
The core technological principle of single-molecule RNA analysis microarrays (RNA microarrays) is to detect RNA directly at the single-molecule level without the need for an amplification process through the use of high-end technologies such as microfluidic chips.
Conventional RNA analysis usually requires reverse transcription and amplification, which can introduce bias and loss of information. Single-molecule RNA microarrays, on the other hand, avoid the problems introduced by amplification by enabling highly accurate detection of RNA.
The technology allows real-time monitoring of the presence and amount of RNA through advanced imaging techniques and biosensors, providing scientists with high-resolution data.
Application Areas：
Cancer Diagnosis and Treatment Monitoring: The RNA chip holds immense potential in cancer research, enabling highly accurate detection of RNA in cancer cells to support early diagnosis and treatment planning.
Infectious Pathogen Detection: Used for detecting RNA from infectious pathogens, it aids in early identification of infection cases, enhancing the effectiveness of infectious disease control.
Neurological Disease Research: High-resolution detection of RNA related to the nervous system contributes to understanding the mechanisms of neurodegenerative diseases and supports new drug development.
Personalized Medicine: Real-time monitoring of individual gene expression provides precise data for personalized medicine, aiding in the formulation of tailored treatment plans.
III. Advantages:
High Sensitivity: Detection at the single-molecule level enhances sensitivity, enabling the discovery of low-concentration RNA molecules.
High Resolution: Provides detailed RNA information, aiding in a comprehensive understanding of gene expression details, including heterogeneity and dynamic changes.
Real-Time Monitoring: Capable of real-time monitoring of the presence and quantity of RNA, facilitating the understanding of real-time dynamic changes in biological processes.
Avoidance of Amplification Bias: No need for RNA amplification, avoiding the introduction of information bias and distortion.
High Throughput: Capable of processing a large number of samples in a short time, enhancing efficiency for high-throughput biological research.
IV. Challenges:
Complex Sample Handling: Overcoming technical challenges in handling complex samples, such as whole blood or tissue samples.
Standardization and Validation: Establishing standardized operating procedures and validation methods to ensure result reproducibility and reliability.
Cost: The preparation and operation of such RNA diagnostic chips may involve higher costs, necessitating considerations for cost-effectiveness during widespread application.
Ethical and Privacy Concerns: High-precision detection of individual gene information may raise ethical and privacy concerns, requiring careful consideration.
V. Future Prospects:
The single-molecule RNA analysis chip continues to evolve technically and in its applications. In the future, researchers may enhance its applicability by optimizing technical processes and addressing challenges. Additionally, with advancements in bioinformatics and artificial intelligence, the chip's ability to handle and analyze vast amounts of single-molecule RNA data will further propel its widespread clinical application. Technological progress will accelerate our understanding of biology and medicine, laying the foundation for precision medicine.
Dxfluidics Products
| Product Code | Outline （mm） | Cross-channel | Thickness （mm） | Chip material | Price （CNY） | ||
| Height （um） | Width1 （um） | Width2 （um） | |||||
| F0001 | 25*46 | 30 | 40 | 100 | 4+1 | PDMS+Glass | 500 |
| F0002 | 25*46 | 30 | 80 | 80 | 4+1 | PDMS+Glass | 500 |
| F0003 | 25*46 | 30 | 80 | 80 | 4+1 | PDMS+Glass | 500 |
| Product Code | Outline （mm） | Height （um） | small cavity （um） | Thickness （mm） | Chip material | Price （CNY） |
| F0004 | 21*35 | 50 | ∮220 | 4+1 | PDMS+Glass | 500 |
| F0005 | 25*10 | 30 | 50*50 | 4+1 | PDMS+Glass | 500 |
| F0006 | 65*21 | 100 | ∮260 | 4+1 | PDMS+Glass | 500 |
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